BLI: Bioluminescence intensity CFB: Cardiac fibroblast GelMA: Gelatin methacryloyl LDH: Lactate dehydrogenase. The latter points to a continuous loss of cells. Negative slopes for the 10 and 15% GelMA constructs point to increasing viability of the cells, while constructs printed using 20 and 25% GelMA show near zero or positive slopes. Right panel: daily change in LDH release expressed as a slope between LDH and number of days in culture. CAD software for 2D drawing, 3D design and 3D printing Efficiency and productivity Expand your visual communication expertise with the precision of superior drafting and design tools available in CorelCAD 2018. (B) Left panel: values of LDH release into the media from the same 3D printed constructs (arbitrary fluorescence units). CorelCAD 2018 provides flexible expansion options for both new users and existing customers to match any workflow. Asterisks stand for difference with 10% GelMA constructs *p < 0.05, **p < 0.01, ***p < 0.001. Positive slopes for the 10 and 15% GelMA constructs point to progressive increase in BLI, while constructs printed with 25% GelMA show decline in BLI during 1 week in culture. Right panel: data shown in left panel recalculated as a daily change in BLI. The BLI values are expressed in ph/s/cm2/sr or photons per second per centimeter squared per steradian. (A) Left panel: values of BLI from 3D printed constructs containing CFBs transfected with luciferin luciferase.
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